A versatile software–programmable microfluidic platform for auto- mated biology. Electronic Supplementary Information

A label-free and enzyme-free platform with a visible output for constructing versatile logic gates using caged G-quadruplex as the signal transducer† †Electronic supplementary information (ESI) available: Experimental details and supplementary figures. See DOI: 10.1039/c7sc04007e

Micro/Nanoscale Parallel Patterning of Functional Biomolecules, Organic Fluorophores and Colloidal Nanocrystals

We describe the design and optimization of a reliable strategy that combines self-assembly and lithographic techniques, leading to very precise micro-/nanopositioning of biomolecules for the realization of micro- and nanoarrays of functional DNA and antibodies. Moreover, based on the covalent immobilization of stable and versatile SAMs of programmable chemical reactivity, this approach constitu...

End-to-end automated microfluidic platform for synthetic biology: from design to functional analysis

BACKGROUND Synthetic biology aims to engineer biological systems for desired behaviors. The construction of these systems can be complex, often requiring genetic reprogramming, extensive de novo DNA synthesis, and functional screening. RESULTS Herein, we present a programmable, multipurpose microfluidic platform and associated software and apply the platform to major steps of the synthetic bi...

Microfluidic Single-cell Culture Chip for Individual Trapping, Cultivation and Selective Release of Yeast Cells

A microfluidic cell-culture chip is presented that enables cultivation and release of selected individual cells. Single cells are trapped in a microfluidic channel using mild suction at defined sites, where they are cultivated under controlled environmental conditions. Cells of interest can be selectively released for further downstream analysis by applying a negative dielectrophoretic force vi...

Field Programmable Gate Array–based Implementation of an Improved Algorithm for Objects Distance Measurement (TECHNICAL NOTE)

In this work, the design of a low-cost, field programmable gate array (FPGA)-based digital hardware platform that implements image processing algorithms for real-time distance measurement is presented. Using embedded development kit (EDK) tools from Xilinx, the system is developed on a spartan3 / xc3s400, one of the common and low cost field programmable gate arrays from the Xilinx Spartan fami...